|In order to study the genetic variability based on the physiologic and drought tolerance indices of 20 genotypes of durum wheat, an experiment was carried out in research farm and physiology laboratory of Agricultural and Natural Resources Campus of Razi University of Kermanshah, Iran, in a randomized complete block design with three replications under stress and non-stress conditions in 2013-2014. The results of analysis of variance for different agronomic, physiological and biochemical traits showed that the effect of genotype on the characteristics of water stored in cut leaves, water use efficiency, stomata conductance, chlorophyll b, total chlorophyll, grain yield, activity of catalase enzymes, peroxidase and superoxide dismutase were significant. Comparison of mean of different traits showed that genotypes 20, 5, 3, 1, 12 and 13 had the highest values. The correlation analysis in drought stress revealed that the grain yield with chlorophyll b and total chlorophyll as well as relative water content had a significant positive correlation with photochemical efficiency of photosystem II. Significant negative correlation was observed between the water stored trait of cut leaves and the level of activity of the enzyme superoxide dismutase. There was a significant positive correlation between chlorophyll a content with chlorophyll b and chlorophyll content as well as between chlorophyll b and total chlorophyll. The GGE-BIPLOT showed that the genotypes 5, 6, 20, 9, 2, 3, 10, 4, and 19 were identified as superior genotypes for studied traits. Such comprehensive information on genotypes is important for converting them to cultivars and introducing them to farmers, as well as choosing parents for hybridization.
1. Ahmadi, A., Y. Emam and M. Pessarakli. 2010. Biochemical changes in maize seedling exposed to drought stress conditions at different nitrogen levels. J. Plant Nutr. 33: 541-556.##Ahmadizadeh, M., A. Nouri, H. Shahbazi and M. Habibpour. 2011. Effects of drought stress on some agronomic and morphological traits of durum wheat (Triticum durum Desf.) landraces under greenhouse condition. Afr. J. Biotechnol. 10: 14097-14107.##Amiri, F. R. and M. T. Assad. 2005. Evaluation of three physiological traits forselecting drought resistant wheat genotypes. J. Agric. Sci. Technol.7: 81-87.##Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts [DOI:10.5897/AJB11.2322
2. polyphenol-oxidase in Beta vulgaris. Plant Physiol. 24: 1-15.##Ashraf, M. Y., A. R. Azmi, A. H. Khan, S. A. Ala. 1994. Effect of water stress on total phenols, peroxidase activity and chlorophyll content in wheat. Acta Physiol Plant. 16(3): 185-191.##Barrs, H. D. 1968. Determination of water deficits in plant tissues. In: T.T. Kozolvski (Ed.), Water Deficits and Plant Growth. Academic Press. 1: 235-368.##Beauchamp, C. and I. Fridovich. 1971. Superoxide dismutases: improved assays and an assay predictable to acrylamide gels. Annals of Biochemistry. 44: 276-287.##Chance, B. and A. C. Maehly. 1995. Assay of catalase and peroxidase. PP. 764-765 In: Culowic, S. P. and Kaplan, N. O. (Eds). Methods in Enzymology Vol. 2. Academic Press. Inc. New York.##Hamrahi, S., D. Habibi, H. Madani, and M. MashhadiAkbarBoojar. 2008. Effect of cycocel and micronutrients on antioxidants rates as indices of drought resistance of rapeseed. New Findings Agric. 2(3): 316-329. (In Persian with English abstract).##Clarke, J. M. and T. N. McCaig. 1982. Excised- leaf water retention capability as an indicator of drought resistance of Triticum genotypes. Can. J. Plant Sci. 62: 571-578.## Egert, M. and M. Tevini. 2002. Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Alliu choenoprasum). Environ. Exp. Bot. 48: 43-49.##Eivazi, A. and F. Habibi. 2013. Water use efficiency variation and its components in wheat cultivars. Am. J. Exp. Agric. 3(4): 718-730.##Farshadfar, E. 2012. Application of integrated selection index and rank sum for screening drought tolerant genotypes in bread wheat. Int. J. Agric. Crop Sci. 4 (6): 325-332.##Farshadfar, E. 2010. New Discussions in Biometrical Genetics (2th Ed.). Islamic Azad University of Kermanshah Publication. (In Persian).##Fayaz, N., and A. Arzani. 2011. Moisture stress tolerance in reproductive growth stage in triticale (TriticosecaleWittmack X) cultivars under field conditions. Crop Breed. J. 1(1): 1-12.##Gale, A., J. Csiszar, I. Tari and L. Erdei. 2002. Change in water and chlorophyll fluorescence parameters under osmotic stress in wheat cultivars. Proceedings of the 7th Hungarian Congress on Plant Physiology, 1 Jan 2002, S2-P05. Acta Biologica Szegediensis, 46(3-4): 85-86.##Gregersen, PL. and P. B. Holm. 2007. Transcriptome analysis of senescence in the flag leaf of wheat. Plant Biotechnol. J. 5: 192-206.##Heidari Sharifabad, H. 2008. Drought mitigation strategies for the agriculture sector. The 10th Iranian Congress of Crop Science. Aug. 18-20, 2008. SPII, Karaj, Iran. (In Persian with English abstract).##Karami, E., M. R. Ghanadha and M. Mardi. 2005. Evaluation of drought tolerance in barley. Iran. J. Agric. Sci. 36 (3): 547-560. (In Persian with English abstract). ##Kaviani, R., M. Aghai Sarbarzeh, M. R. Bihamta and M. Mohammadi. 2013. Genetic diversity and factor analysis for agronomical and morphological traits in durum wheat landraces. Seed Plant Improv. J. 29(4): 673-692. (In Persian with English abstract). ##Kazemi, F., D. Habibi, E. Fathollahzade, D. Taleghani, M. Mashhadiakbar Bujar, and H. Jalilevand, 2008. Effect of drought stress on yield, yield componentsand antioxidant enzyme activity in different cultivars of red beans. Agric. Res.1 (1): 81-93 (in Farsi with English Summary).##Khadem Pir, M., S. Galeshi, A. Soltani and F. Ghaderi-Far. 2015. Effect of flood duration and nitrogen-fed type on quantitative and qualitative traits of soybean. J. Plant Prod. Res. 22 (3). (In Persian with English abstract).##Lichtenthaler, H. and A. R. Wellburn. 1983. Determination of total carotenoids and chlorophyll a and chlorophyll b leaf extracts in different solvents. Biochem. Soc. Transactions. 603: 591-592.##Mohammadi, M., R. Karimizadeh and M. Abdipour. 2011. Evaluation of drought tolerance in bread wheat genotypes under dryland and supplemental irrigation conditions. Aust. J. Crop Sci. 5 (4): 487-493.##Mohammadi, R. and E. Farshadfar. 2003. Determining chromosomes controlling physiological traits associated with drought tolerance in wheat. Iran. J. Crop Sci. 5 (2): 117-132. (In Persian with English abstract).##Ramachandra Reddy, A., K. V. Chaitanya, P. P. Jutur and K. Sumithra. 2004. Differential antioxidative responses to water stress among five mulberry (Morus alba L.) cultivars. Environ. Exp. Bot. 52(1): 33-42.##Renu, K. C. and S. Devarshi. 2007. Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than susceptible wheat cultivar under field conditions. Environ. Exp. Bot. 60: 276-283.##Romena, M. H. 2019. Genetic analysis of some drought tolerance agronomical, physiological and metabolical criteria in some wheat cultivars at in-vivo and in-vitro conditions. Ph.D thesis, Razi University, Kermanshah, Iran.##Rubio, J., J. I. Cubero, L. M. Martin, M. J. Suso and F. Flores. 2004. Biplot analysis of trait relations of white lupin in Spain. Euphytica.135: 217-224.##Sayar, R., H. Khemira, A. Kameli and M. Mosbahi, 2008. Physiological tests as predictive appreciation for drought tolerance in durum wheat. Agron. Res. 6 (1): 79 - 90.##Sinha, A. K. 1972. Colorimetric assay of catalase. Analytic. Biochem. 47 (2): 389-394.##Shao, H. B., Z. S. Liang, M. A. Shao and B. C. Wang. 2005. Changes of some
3. physiological and biochemical indices for soil water deficits among 10 wheat genotypes at seedling stage. Colloids Surfaces B: Biointerfaces, 42 (1): 107- 113.##Yan, W., and I. R. Rajcan. 2002. Biplot analysis of test sites and trait relations of soybean in Ontario. Can. J. Plant Sci. 42: 11-20.##Yan, W. and J. Fregeau-Reid. 2008. Breeding line selection based on multiple traits. Crop Sci. 48: 417-423.##Yan, W. and N. A. Tinker. 2005. An integrated biplot analysis system for displaying, interpreting and exploring genotype × environment interaction. Crop Sci. 45: 1004-1016.##Yan, W., L. A. Hunt, Q. Sheng and Z. Szlavnics. 2000. Cultivar evaluation and mega-environment investigation based on the GGE biplot. Crop Sci. 40: 597-605.##