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Identification of molecular markers linked to Lr34/Yr18 gene and evaluation of resistance to leaf rust and yellow rust in wheat (Triticum aestivum L.) cultivars and promising lines
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Habibollah Ghazvini   , Mohsem Sarhangi , Farzad Afshari |
| Associate Prof., Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran |
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Abstract: (3334 Views) |
| Leaf rust (Puccinia triticina) and yellow rust (Puccinia striiformis) are known as the most important and prevalent diseases of wheat worldwide, causing significant losses in the world’s wheat production. Transfer of leaf and yellow rusts resistance genes to wheat cultivars is one of the most economical and effective methods to control of diseaseses. Lr34/Yr18 is a resistance gene which confers a durable resistance to wheat leaf and yellow rusts. In the recent years, several molecular markers linked to Lr34/Yr18 have been identified. In this experiment, presence of Lr34/Yr18 in some Iranian wheat cultivars and promising lines was evaluated under field condition along with some recently developed molecular markers. Evaluation of plant resistance of wheat genotypes to yellow rust and leaf rust was conducted under artificial inoculation in disease nurseries of Cereal Research Department, SPII, Karaj and Golestan station in Ahvaz, Iran respectively, in 2014. Results of molecular assays indicated that three markers caSNP4, caSNP12 and Cssfr1 predicted presence of Lr34/Yr18 with more confidence compared to csLV34. These markers may be easily used in the genetic laboratories with limited equipments. Based on the results of caSNP4 and caSNP12 markers, from 85 wheat genotypes, 18 genotypes had linked alleles to Lr34/Yr18 gene. Comparison of the field evaluation with results of molecular assay indicated that most of the wheat genotypes with Lr34 gene had resistance and moderately resistant to moderately susceptible infection responses to leaf rust and yellow rust. |
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| Keywords: Adult plant resistance, Codominant marker, Marker assisted selection, Molecular breeding and Wheat. |
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Full-Text [PDF 928 kb]
(1862 Downloads)
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2018/10/27 | Accepted: 2018/10/27 | Published: 2018/10/27
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