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Nooshafrin Karami   , Ali Aalami , Habibollah Samizadeh , Mehrzad Alahgholipour , Babak Rabiei |
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Abstract: (4387 Views) |
| One of the important factors determining the rice grain quality is fragrance. In this experiment, 29 Iranian rice cultivars were analyzed for aroma using qualitative test, 1.7% KOH sensory test on leaves and grains and molecular methods based on specific markers at Rice Research Institute of Iran, Rasht, Iran in 2011. Qualitative fragrance test divided rice varieties into two fragrant and non-fragrant categories 13 fragrant and 16 non-fragrance cultivars were identified. Superior fragrant cultivars were Domsiah, Alikazemi, Binam, Fajr and Hashemi and the most non-fragrant cultivars identified as Nemat, Khazar, Neda, Sepidroud and Dasht. Molecular evaluation results based on SSR4463-L413 and allele specific amplification (ASA) markers indicated that SSR4463-L413 marker was polymorphic in fragrant and non- fragrant cultivars. SSR4463-L413 marker also identified 13 fragrant and the others as non-fragrant cultivars. This finding was similar to qualitative test of aroma. ASA marker also identified 13 cultivars fragrant and the others as non-fragrant. Results of this study indicated that SSR4463-L413 marker can discriminate aromatic and non-aromatic Iranian rice cultivars with high resolution, therefore, it can be used as an efficient marker for evaluating and fast screening of genotypes in the rice segregating population. Also, the results indicated that ASA marker, in addition to ability in predicting homozygous aromatic and non-aromatic cultivars, it could identify heterozygous non-aromatic individual rice plants therefore, it can be suggested as a co-dominant marker for selection in rice segregating population for aroma. |
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| Keywords: 2-acetyl-1-pyrroline, Betaine aldehyde dehydrogenase, Rice, Specific Allele and Specific marker. |
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Full-Text [PDF 508 kb]
(2550 Downloads)
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/02/15 | Accepted: 2015/02/15 | Published: 2015/02/15
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