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Development of PCR based marker for candidate Fusarium head blight resistance gene (PDR5) in wheat
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Forooh Ehya   , Mohammad Reza Ghaffari , Mohsen Mardi , Saburan Zaheri , Behzad Gharehyazi |
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Abstract: (3962 Views) |
| The PDR5 (pleiotropic drug resistance 5) gene, encoding ABC transporter protein, detoxifies cells using ATP hydrolysis. To develop PCR based marker for Fusarium head blight resistance candidate gene (PDR5) in wheat, similar sequences of PDR5 were aligned with DNASTAR software. Primers were designed based on conserved and non conserved sequences. Molecular analysis using moderately resistant (Frontana) and susceptible (Falat) parental lines showed a specific PDR5 allele in Frontana. Amplified allele was isolated, cloned and sequenced. The sequencing was verified on data Bank. Single marker analysis based on phenotyping and genotyping data of F3 generation developed from a cross between Frontana and Falat indicated the specific PDR5 allele had negative effect on FHB infection in wheat. |
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| Keywords: Wheat, Fusarium Head Blight, PCR, PDR5 gene, Phenotyping, Genotyping |
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Full-Text [PDF 357 kb]
(1460 Downloads)
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/03/1 | Accepted: 2015/03/1 | Published: 2015/03/1
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