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Assessment of genetic diversity in alfalfa (Medicago sativa L.) ecotypes from central and eastern regions of Iran using SSR markers
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Abstract: (3775 Views) |
| Genetic variation in 33 alfalfa ecotypes from central and eastern regions of Iran was assessed using SSR markers. PCR analysis was performed on genomic DNA using 10 microsatellite primer pairs. PCR products were detected using denatured polyacrylamide gel. From 62 reproducible bands identified, 52 bands were polymorphic corresponding to an average of 5 bands per locus. The proportion of polymorphism with an average of 80.64% varied from 62.5 for MTIC250 to 100 for ENOD 20. The average of polymorphism information content (PIC) was 0.61. ENOD20 and MAL369471 primers had the highest PIC value (0.78 and 0.83) and the lowest one (0.37 and 0.34) in central and eastern ecotypes, respectively. Nei’s gene diversity and Shannon’s information index for both of populations were calculated and their values were 0.29, 0.3 and 0.45, 0.46, respectively. Genetic similarity was estimated using the Dice coefficient, and dendrogram was constructed using the UPGMA method. Generally, results showed that despite of the similarities of genetic structures between the ecotypes from two regions, high variation was observed among individual plants possibly due to the high allogamy (90%), insects activity (particularly bees) and pollen transmission among ecotypes. Genetic similarity between ecotypes in this study could be considered as consequeneces of genetic equilibrium that have been occurred over long time of cultivation of alfalfa in these regions as well as seed exchange among provinces. |
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| Keywords: Alfalfa, Genetic variation and Microsatellite marker |
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Full-Text [PDF 325 kb]
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/02/22 | Accepted: 2015/02/22 | Published: 2015/02/22
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