To determinate the optimum combination of barley with fenugreek in intercropping at different cow manure levels, a field experiment was carried out as split plot arrangements in randomized complete blocks design with three replications at research farm of Ramin Agriculture and Natural Resources University of Khouzestan, Iran in 2014-2015 growing season. Four manure levels including; 0, 12, 24 and 36 t.ha^-1 were assigned to main plots and eight levels of replacing and addative ratios of intercropping including; 75% barley + 25% fenugreek, 50% barley + 50% fenugreek, 25% barley + 75% fenugreek, 100% barley + 16.6% fenugreek, 100% barley + 33.3% fenugreek, 100% barley +50% fenugreek and pure stands of barley and fenugreek were randomized in sub-plots. Results showed that the highest dry matter yield (3166 kg.ha^-1) obtained from 100% barley + 50% fenugreek with 36 t.ha^-1 of manure. The highest crude protein content and dry matter digestibility obtained in pure fenugreek with 36 t.ha^-1 of manure. The highest levels of total carbohydrate obtained from pure barley with 24 t.ha^-1 of manure. Results of this experiment showed that 100% barley + 50% fenugreek with 36 t.ha^-1 of manure produced the maximum dry forage yield and quality and the highest LER (1.180), therefore was identified as superior treatment.