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Assessment of inheritance of resistance to root-knot nematode (Meloidogyne javanica) race 1 in common bean (Phaseolus vulgaris L.) using generations mean analysis
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Leila Sadeghi Azghan   , Masoud Naderpour , Salar Jamali , Mohammad Reza Jazayeri Nooshabadi , Habibollah Samizadeh Lahiji , Mohammadreza Naghavi , Mohammadreza Jazayeri Noushabadi |
| Agricultural Research, Education and Extension Organization (AREEO) |
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Abstract: (23 Views) |
Introduction: Common bean (Phaseolus vulgaris L.) is one of the important crops of Leguminosae with valuable sources of proteins, fiber, carbohydrates and mineral elements necessary for human diet (Lisciani et al., 2024). However, significant numbers of biotic and abiotic stresses including plant pathogenic root-knot nematodes (Meloidogyne spp.) affect its production (Jones et al., 2013) that necessitate breeding programs to improve bean resistance for sustainable production. Resistance to Meloidogyne spp. often inherits by dominant and recessive genes, affects their reproduction, and support lower population of invading nematodes (Roberts, 2022). In the present study, genetic analysis of resistance against M. javanica race 1 population in common bean cv. Blanco and cv. Alabama #1 of CIAT and the mode of inheritance was studied.
Materials and Methods: Segregation of resistance gene(s) against M. javanica race 1 was studied in parentals, F1, F2-1, F2-2, BC1-R, and BC1-S generations derived from crosses between the cv. Blanco (susceptible parent) × cv. Alabama #1 (resistant parent) in common beans. The experiments were performed using completely randomized design under greenhouse conditions of Seed and Plant Certification and Registration Research Institute, Karaj, Iran, in 2018-2020. Root galling intensity and egg mass production were measuered and counted following the nematode inoculation.
Results: The results of analysis of variance and means comparison among generations indicated significant difference in phenotypic values between the susceptible parent (cv. Blanco) and the resistant parent (cv. Alabama #1), as well as between cv. Blanco and the F1 generation. However, no significant difference was observed between cv. Alabama #1 and the F1 generation. The phenotypic values of F2 and back cross populations were greater than those of cv. Alabama #1 and were more similar to those of cv. Blanco, indicating relative dominance toward susceptibility. The susceptibility vs. resistance phenotypes to the nematode in F2 population corresponded to the ratio 3:1 that was consistent with those of the generation means analysis. The variance component analysis revealed that the variance of the dominance effects of egg mass and galling indices exceeded that of the additive ones. Dominant genes effect were predominantly found in the susceptible parent, which exhibited higher gall index and egg mass than those of the resistant parent.
Conclusion: The findings of this experiment indicated that selecting resistant lines of P. vulgaris against M. javanica race 1 was not feasible in the early generations due to the instability of the genetic material. Therefore, such efforts should be conducted in the advanced breeding generations.
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| Keywords: Common bean, Galling index, Egg mass index, Phenotypic value, Resistance genes segregation and Root-knot nematode |
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Full-Text [PDF 1014 kb]
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2025/03/14 | Accepted: 2025/09/5 | Published: 2025/10/27
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