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Mapping of an ABC-Transporter gene associated with barley scald disease (Rhynchosporium secalis (Oud.) Davis) using a CAPS marker
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Abstract: (4894 Views) |
| ABC-Transporter proteins superfamily are found in all alive organisms as connection bridge in cellular membranes. These proteins are responsible for transportation of variant substrates such as metabolites that involved in plant defense mechanisms. In this study SNPs and CAPS markers were used for mapping of an ABC-Transporter which is specific for compatible and incompatible interaction against R. secalis fungus. A 2.2kb fragment derived from PCR product with specific primers for mentioned gene was sequenced in 10 barley parents. Among observed SNPs, one SNP showed different restriction enzymes sites in Chebec and Harrington parents and converted to CAPS marker. Results mapping revealed position of the gene on long arm of barley chromosome 3. Also, a homology evaluation for this region of chromosome was in accordance with previous studies about high synteny for barley chromosome 3 with rice chromosome 1. |
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| Keywords: Barley, Gene Mapping, ABC-Transporter, SNP, CAPS, NlaIII, Synteny, Scald |
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/02/28 | Accepted: 2015/02/28 | Published: 2015/02/28
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