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Analysis of genetic diversity in barley genotypes using SSR and EST-SSR markers
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Abstract: (4021 Views) |
| Determination of genetic diversity level and genotype relationships is fundamental for identification of desirable parents to be used in different breeding programs. Molecular markers have been successfully used for analysis of genetic diversity in various crops. Genetic diversity among 35 lines and genotypes of barley was studied using 19 pairs of SSR and EST-SSR primers. Totally 157 alleles were produced with an average of 4.06 alleles per locus. Bmag581 with two and Bmag134 with 15 alleles had the lowest and highest allele number, respectively. Polymorphic information content ranged from 0.15 (Bmag518) to 0.89 (Ebmac419) with an average of 0.69. The markers Bamg581 and Ebmac419, had the lowest (0.17) and the highest (0.90) gene diversity, respectively. Cluster analysis based on molecular data assigned the genotypes into five groups. Group 2 genotypes showed low level of tolerance to cold and contained sensitive genotypes. In general, it seems that the studied markers were able to differentiate the most cold sensitive barley genotypes from the rest. |
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| Keywords: Barley, Cluster analysis, EST-SSR markers and Genetic diversity |
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Full-Text [PDF 139 kb]
(1211 Downloads)
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/02/17 | Accepted: 2015/02/17 | Published: 2015/02/17
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