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Evaluation of reaction of common bean (Phaseolus vulgaris L.) genotypes to bean common mosaic necrosis virus (BCMNV) disease in greenhouse conditions
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Abstract: (3865 Views) |
| This experiment was conducted to determine common bean genotypes reaction to Bean Common Mosaic Necrosis Virus (BCMNV). Twenty five common bean genotypes were obtained from National Bean Research Station of Khomein. These genotypes were planted using randomized complete block design with three replications, and inoculated with inoculums of virus in greenhouse conditions in 2009. The reactions of plants were examined twenty one days after planting using ELISA test and IC-RT-PCR and visual methods. Measured traits in this research included: leaf infection (actual infection), mosaic leaf, systemic necrosis, vein strip, chlorotic spot and leaf shape. Quality traits (except leaf infection) were assessed by visual ranking method (visual symptom). Results indicated that genotypes were significantly different in leaf infection (actual infection), leaf mosaic, systemic necrosis and leaf shape. Results also showed that Ks-21189, Ks-21481 and Derakhshan were susceptible and COS16, Goli and Ks-41128 were resistant genotypes. Systemic necrosis had positive and highly significant correlation (r=0.638**) with leaf infection. Results of cluster analysis also indicated that Ks-21189 and COS16 as sensitive and resistance genotypes, respectively. Chi-square test indicated that white genotypes were more resistant than pinto and red genotypes. IC-RT-PCR results indicated that BCMNV virus was present in Ks-21189 genotype. |
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| Keywords: : Common bean, BCMNV, ELISA, IC-RT-PCR, Resistance and Virus |
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Full-Text [PDF 634 kb]
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/02/16 | Accepted: 2015/02/16 | Published: 2015/02/16
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