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Effect of drought stress on compatible osmolytes content, enzyme activity and grain yield in chickpea (Cicer arietinum L.) genotypes
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Abstract: (4228 Views) |
| The relationship between accumulation of compatible osmolytes and superoxide dismutase (SOD) activity with grain yield of chickpea genotypes in responses to drought stress was studied in two separate experiments a field and a pot experiments at the Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran, in 2010-11 cropping season. Field experiment was carried out as split plot arrangement in randomized complete blocks design (RCBD) with three replications. Irrigation and rainfed conditions were assigned to main plots and chickpea genotypes were randomized in subplots. Pot experiment was conducted as factorial arrangement using RCBD with two factors including irrigation treatments (irrigation at -3 (control) and -12 bar (stress) of soil water potential) and 19 chickpea genotypes as second factor with three replications. Results showed that the effects of drought stress and genotype on grain yield was significant in the field experiment. Genotypes showed different responses to drought stress. In pot experiment some physiological traits showed specific relationship with genotype performance under field condition. In the reproductive stage, drought stress decreased leaf soluble protein from 17.5 to 5 mg.g-1. However, there was no relationship between concentration of total soluble leaf protein and drought tolerance, but increasing of proline and soluble carbohydrates concentration, improved stress tolerance index especially in the vegetative stage. Results showed positive relationship between accumulation of proline and soluble carbohydrates concentration (r = 0.59**), that revealed a common role of these osmolytes in improvement of drought stress tolerance in chickpea genotypes. In the vegetative stage, drought stress increased proline concentration from 0.27 to 2.4 mg.g-1 and soluble carbohydrates from 14 to 16.9 mg.g-1. These results indicated that soluble carbohydrates accumulation had stronger relationship with drought tolerance rather than proline concentration, and soluble carbohydrates had two fold higher osmotic adjustment than proline. It can be concluded that accumulation of soluble carbohydrates is a better index in selection for drought tolerance in chickpea. However, results showed no relationship between SOD activity and stress tolerance in chickpea genotypes. |
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| Keywords: Chickpea, Drought stress, Leaf soluble Carbohydrate and Superoxide dismu |
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Full-Text [PDF 573 kb]
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/02/15 | Accepted: 2015/02/15 | Published: 2015/02/15
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