[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 6, Issue 2 (7-2004) ::
علوم زراعی 2004, 6(2): 0-0 Back to browse issues page
High frequency plantlet regeneration in the dioecious plant sorrel
Abstract:   (3698 Views)
Sorrel [Rumex acetosa L. (Polygonacea)] is a dioecious perinnial species which is being used as a model plant in sex determination molecular genetic studies. In this study for determination of the most suitable explant and medium for regeneration in this species, explants from different organs such as leaf, petiol, cotyledon, hypocotyle and root were cultured on Murashing and Skoog (MS) medium supplemented with vitamins, 2% sucrose, 0.8% agar and different compositions of hormons. Auxins IAA, NAA and cytokenins Zeatin, BAP, TDZ and Kinetin as well as IAA and BAP with Gibberllic Acid were added to culture media. Experiments were carried out as factorial in completely randomized design. Callus induction and regeneration from explants were evaluated. The most suitable explants and medium for regeneration were leaf and MS medium supplemented with 0.75 mgl-1 IAA and 1.5 mgl-1 BAP, respectively. The highest callus induction and regeneration was obtained from the leaf explants at the rate of 100% and 81%, respectively. Regenerates from the leaf explants on this medium had proliferated status. Histological studies confirmed that the origin of regeneration was from active and condensed cells that had developed from central parts of callus followed by marginal cells to form single or proliferated primordial. These results suggest new, simple, short and efficient regeneration method in this model species which can be used in genetic studies and gene transformation.
Keywords: Sorrel (Rumex acetosa L), Explant, Callus induction, Regeneration, Media, Plantlet
Full-Text [PDF 285 kb]   (1003 Downloads)    
Type of Study: Scientific & Research | Subject: Special
Received: 2015/03/1 | Accepted: 2015/03/1 | Published: 2015/03/1
Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

High frequency plantlet regeneration in the dioecious plant sorrel. علوم زراعی 2004; 6 (2)
URL: http://agrobreedjournal.ir/article-1-356-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 6, Issue 2 (7-2004) Back to browse issues page
نشریه علوم زراعی ایران Iranian Journal of Crop Sciences
Persian site map - English site map - Created in 0.05 seconds with 37 queries by YEKTAWEB 4645