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:: Volume 19, Issue 4 (winter 2018) ::
علوم زراعی 2018, 19(4): 334-348 Back to browse issues page
Mapping chromosome locations of two genes independently controlling stem rust resistance in wheat (Triticum aestivum L.) using haplotype analysis and genetic modeling
Abstract:   (3014 Views)
In spite of various methods which have been developed to identify and map quantitative trait loci (QTL) on genomes, mapping chromosome locations of two independent genes simultaneously controlling a qualitative trait in a genetic population have not been reported. In this study, F2 population derived from a cross between RL6071 (susceptible) and Tr129 (resistant) was inoculated with spores of Puccinia graminis Pers. causal agents of stem rust in wheat and population was classified into resistance and susceptible groups. Phenotypic assessment of F2 and F2:3 populations showed that there was a 15:1 segregation ratio for stem rust resistance indicating presence of two dominant resistance genes independently controlling this trait. Four hundred twenty two SSR markers which had a good coverage for all 21 chromosomes of wheat were used to evaluate the correspondences of alleles between bulked DNA from susceptible lines with those of both parents. Markers, showing good correspondence of alleles between bulked susceptible lines with susceptible parent, were used to compare alleles of susceptible parent RL6071 with those of 161 F2 progenies. Using segregation of alleles for SSR markers in F2 population and genetic modeling, the presence of two dominant resistance genes independently controlling rust resistance in Tr129 was confirmed. Based on genetic analysis of data and haplotype analysis of linked markers to each gene, the stem rust resistance genes in line Tr129 were mapped on chromosomes 6A and 2B of line Tr129.   
Keywords: Haplotype analysis, Genetic modeling, Stem rust of wheat, SSR markers and Genetic maps
Full-Text [PDF 476 kb]   (2212 Downloads)    
Type of Study: Scientific & Research | Subject: Special
Received: 2018/02/28 | Accepted: 2018/02/28 | Published: 2018/02/28
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Mapping chromosome locations of two genes independently controlling stem rust resistance in wheat (Triticum aestivum L.) using haplotype analysis and genetic modeling. علوم زراعی 2018; 19 (4) :334-348
URL: http://agrobreedjournal.ir/article-1-823-en.html


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Volume 19, Issue 4 (winter 2018) Back to browse issues page
نشریه علوم زراعی ایران Iranian Journal of Crop Sciences
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