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Evaluation of molecular diversity in rice (Oryza sativa L.) genotypes using microsatellite markers linked with agronomic and grain physico-chemical characteristics
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Abstract: (3869 Views) |
| Ninety-four rice genotypes were evaluated for genetic diversity using 52 rice SSR markers linked with some important agronomic and grain quality characteristics which well distributed on 12 chromosomes of rice. The total number of polymorphic alleles was 361 alleles with the average of 4.750 alleles per SSR locus. Results revealed that some markers such as RM276 and RM5642 on chromosome 6 and RM14 and RM1 on chromosome 1 have more than 9 observed alleles. The highest and lowest PIC values were observed for primers RM5642 (0.854) and RM207 (0.515), respectively. Cluster analysis using complete linkage method based on Jaccard similarity coefficient for molecular data showed that all genotypes were classified in nine clusters. Nine cultivars/lines from different cluster including Daylamani, Tarommohali, Line 23, RI1843046 (Saleh×Hashemi), RI1843223 (Saleh×Mohamadi), IR50, Back cross line [(Saleh×Abjiboji) ×Abjiboji], RI184421 (Sepidrod×Hassansaraiee) and RI184472 (Sepidrod×Gharib) were selected as parents to be used in rice breeding programs. The similarity coefficient was very low among the selected parental cultivars/lines. Therefore, high diversity in breeding populations is expected by using these selected cultivars/lines as parents of crosses for improvement of important agronomic and physico-chemical characteristics. Marker assisted selection as a powerful technique can be used for development of the new rice cultivars because these parental cultivars/lines were recognized by some SSR markers linked with QTLs for agronomic and grain quality characteristics. |
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| Keywords: Cluster analysis, Molecular diversity, Rice, Similarity coefficient and SSR markers. |
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Full-Text [PDF 323 kb]
(1745 Downloads)
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/02/15 | Accepted: 2015/02/15 | Published: 2015/02/15
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