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QTLs mapping of physiological and biochemical traits of barley under drought stress condition
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Abstract: (4013 Views) |
| To map genomic locations controlling physiological and biochemical characteristics of barley under normal and drought stress conditions and to determine their relationships with grain yield an experiment was conducted at Research Farm, College of Agriculture, University of Zabol in 2011.The experiment was carried out using two randomized complete block designs with three replications under non-stress and drought stress conditions. Plant materials were 72 doubled haploid lines andtheir parents (Steptoe and Morex). Physiological and biochemical traits such as grain yield, chlorophyll content, F0, Fm, Fv/Fm, RWC, WSC and proline content were measured. QTL analysis was separately conducted by composite interval mapping (CIM) method for each trait, experiment, and for mean of two experiments. Barley molecular marker linkage map comprised of 327 RFLP markers with 1226.3 cM length and 3.75 cM mean distance. There were significant diffrences among genotypes for all traits and transgressive segregations were also observed in both directions (positive and negative). Eighty five QTLs controlling different traits were identified from which 72 were stable. Phenotypic variances explained by these QTLs varied from 8.28% for proline content to 25.66% for Fm. The highest LOD scores were obtained for Fm on chromosome 2H. Stable QTLs can be used by Marker Assisted Selection (MAS). |
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| Keywords: Barley, Biochemical traits, Drought stress, Mapping, Physiological traits and QTL. |
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Full-Text [PDF 914 kb]
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/02/15 | Accepted: 2015/02/15 | Published: 2015/02/15
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