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The expression analysis of transcription factors under long term salt stress in tolerant and susceptible wheat genotypes using reverse northern blot technique
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Abstract: (4183 Views) |
| Soil salinity is a serious problem in agricultural production systems, particularly where the majority of crop plants have a low level of salt tolerance. The identification of genes whose expression enables plants to adapt or tolerate to salt stress conditions is essential for breeding programs, however little is known about the role of different transcription factors as important regulatory genes in salt stress, specially under long term salinity stress. To elucidate the role of these regulatory factors in response to long term salt stress in two wheat genotypes Chinese Spring (susceptible) and Mahouti (tolerant), eleven transcription factor genes from different families (bZIP, bHLH, WRKY, MYB and NAC) were analyzed by reverse northern bolt under control and stress (250 mM NaCl) conditions. The results showed that the selected genes were affected by salinity in both genotypes and were up- or down-regulated and the variation of expression ratio in Mahouti variety was higher than Chinese Spring. The expression of three transcription factor genes (bHLH2, bZIP5 and MYB3) varied more than two-folds in response to salt stress. These genes were significantly up-regulated only in tolerant genotype, var. Mahouti, indicating that these genes may potentially regulate a long term plant response to salt stress in wheat, and can be considered as suitable candidates for further genetic analysis. It is concluded that the transcription factors can be involved in long term salt stress response of wheat plant, in contrary to general view which distinguishes their contributions only for a short term response to salt stress. |
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| Keywords: Gene expression, Long term salinity, Reverse northern blot, Transcription factor and Wheat. |
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Full-Text [PDF 334 kb]
(2557 Downloads)
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Type of Study: Scientific & Research |
Subject:
Special
Received: 2015/02/17 | Accepted: 2015/02/17 | Published: 2015/02/17
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