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:: Volume 21, Issue 1 (Spring 2019 2019) ::
علوم زراعی 2019, 21(1): 31-44 Back to browse issues page
Effect of seed priming and application of cytokinin and auxin on growth and grain yield of wheat (Triticum aestivum L.) under Ahvaz climatic conditions
Narges Khamdi , Majid Nabipour , Habibollah Roshanfekr , Afraceab Rahnama
Professor, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (3723 Views)
To evaluate the effect of seed priming and application of cytokinin and auxin on grain yield wheat cv. Chamran, an experiment was carried out in Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran, in 2015-16 and 2016-17 growing seasons. This experiment was carried out as factorial arrangement in randomized complete block design with three replications. Experiment was carried out in a factorial arrangement based on randomized complete block design with three replications. Treatments included seed priming (hydropriming and control) and plant growth regulators application (nine cytokinin and auxin application levels at one and two weeks after anthesis). The results showed that hydropriming significantly increased grain yield by about 8% in comparison with control (7030 and 6507 kg.ha-1, respectively). Application of cytokinin and auxin hormones had positive effect on photosynthesis and soluble proteins of flag leaf. In addition, number of endosperm cells was also increased by application of growth regulators. The highest effect on increasing source strength was observed in application of cytokinin in both times of foliar application. Grain filling rate was also significantly highest, about 1.79 mg grain-1 day-1, in cytokinin-cytokinin foliar application, followed by auxin-cytokinin. Grain yield in cytokinin-cytokinin (7891 kg.ha-1) increased by 24.7% in comparison with control. Interaction effect of seed priming × plant growth regulator application was not significant for any traits. Results of this experiment showed that seed priming increased number of spike per.m-2. Foliar application of auxin and cytokinin also may increase grain yield of wheat through enhancing of source and sink strength.

 
Keywords: Endosperm cells, Grain filling, Hydropriming, Plant growth regulators and Wheat
Full-Text [PDF 558 kb]   (1419 Downloads)    
Type of Study: Scientific & Research | Subject: Special
Received: 2019/06/3 | Accepted: 2019/06/3 | Published: 2019/06/3
References
1. Aboutalebian, M. A., F. Sharifzadeh, M. R. Jahansouz, A. Ahmadi, and M. R. Naghavi. 2008. The Effect of seed priming on germination, stand sstablishment and yield of wheat (Triticum aestivum L.) cultivars in three different climates of Iran. Iran. J. Field Crops Sci. 39(1): 145-154. (In Persian with English abstract).##Abdolrahmani, B., K. Ghassemi-Golezani, M. Valizadeh, V. Feizi-Asl, and A. R. Tvakoli. 2011. Effects of seed priming on seed vigor and grain yield of barley (Hordeum vulgare L. cv. Abidar) in rainfed conditions. Seed Plant Prod. J. 11(4): 337-352 (In Persian with English abstract). ##Abdolrahmani, B., K. Ghassemi-Golezani, M. Valizadeh and V. FeiziAsl. 2007. Seed priming and seedling establishment of barley (Hordeum voulgare). J. Food Agric. Environ. 5: 179-184.##Amal, M., E. Shraiy, and A. M. Hegazi. 2009. Effect of acetylsalicylic acid, indole-3-butyric acid and gibberellic acid on plant growth and yield of pea (Pisum sativum L.). J. Basic Appl. Sci. 3: 3514-3523.##Ashraf, M. Y., N. Azhar and M. Hussain. 2006. Indole acetic acid (IAA) induced changes in growth, relative water contents and gas exchange attributes of barley (Hordeum vulgare L.) grown under water stress conditions. Plant Growth Regul. 50:85-90.##Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts: polyphenol-oxidase in Beta vulgaris. Plant Physiol. 24: 1-15.##Bajguz, A. and A. Piotrowska. 2009. Conjugates of auxin and cytokinin. Phytochemistry. 70: 957-969##Bhatia, S. and R. Singh. 2002. Phytohormone-mediated transformation of sugars to starch in relation to the activities of amylases, sucrose-metabolising enzymes in sorghum grain. Plant Growth Regul. 00: 1-8.##Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye bonding. Analytical Biochem. 72: 248-254.##Brenner, M. L. and N. Cheikh. 1995. The role of phytohormones in photosynthate partitioning and seed filling. In: Davies P.J. (Ed.), Plant Hormones. Kluwer Academic Press, Dordrecht, Boston, London, pp. 649-670.##Davies, P. J. 1987. The Plant Hormones: Their Nature, Occurrence and Functions. In: P. J. Davies, P. J. (Ed.), Plant Hormones and Their Role in Plant Growth and Development. Martinus Nijhoff Publishers, The Netherlands, pp 1-11.##Dua, I. S. and S. N. Bhardwaj. 1979. Levels of endogenous growth regulators in wheat during early stages of grain setting and development. Indian J. Agric. Sci. 55: 622-627. ##Dubios, M. K. A. Gills, J. K. Hamilton, P. A. Rebers and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Annals Chem. 28(3): 350-356.##Ellis, R. H. 1992. Seed and seedling vigour in relation to crop growth and yield. Plant Growth Regul. 11: 249-255.##Farooq, M., S. M. A. Basra, H. Rehman and B. A. Saleem. 2008. Seed priming enhances the performance of late sown wheat (Triticum aestivum L.) by improving chilling. J. Agron. Crop Sci. 194: 55-60.##Ghorbani Javid, M., A. Soroushzadeh, S. A. M. Modarres Sanavy, I. Allahdadi and F. Moradi. 2011. Effects of the exogenous application of auxin and cytokinin on carbohydrate accumulation in grains of rice under salt stress. Plant Growth Regul. 65:305-313. ##Giri, G. S. and W. F. Schillinger. 2003. Seed priming winter wheat for germination, emergence and yield. Crop Sci. 43: 2135-2141. ##Gupta, N. K. S. Gupta, D. S. Shukla and P. S. Deshmukh. 2003. Differential responses of BA injection on yield and specific grain growth in contrasting genotypes of wheat (Triticum aestivum L.). Plant Growth Regul. 40: 201-205.##Hakoomat, A., I. Nadeem, S. Naeem, A. Shakeel and M. Athar. 2013. Seed priming improves irrigation water use efficiency, yield and yield components of late-sown wheat under limited water conditions. Turk. J. Agric. Forest. 37: 534-544. ##Harris, D., A. K. Pathan, P. Gothkar, A. Joshi, W. Chivasa and P. Nyamudeza. 2001. On-farm seed priming: using participatory methods to revive and refine a key technology. Agric. Sys. 69: 151-164.##Hashemi Dezfouli, A. And A. S. Marashi. 1995. Changes in photosynthetic material at flowering time and its effect on grain growth, yield and yield components of wheat. J. Agric. Sci. Technol. 9: 32-16. (In Persian with English abstract).##Havlova, M., P. I. Dobrev, V. Motyka, H. Storchova, J. Libus, J. Dobra', J. Malbeck, H. Gaudinova and R. Vankova. 2008. The role of cytokinins in responses to water deficit in tobacco plants over-expressing trans-zeatin O-glucosyltransferase gene under 35S or SAG12 promoters. Plant Cell Environ. 31: 341-353.##Herzog, H. 1982. Relation of source and sink during grain filling period in wheat and some aspects of the regulation. Physiol. Plant. 56: 155-160.##Khan, M. B., M. Gurchani, M. Hussain and K. Mahmood. 2010. Wheat seed invigoration by pre-sowing chilling treatments. Pak. J. Bot. 42: 1561-1566.##Liang, J., J. Zhang and X. Cao. 2001. Grain sink strength may be related to the poor grain filling of indica-japonica rice (Oryza sativa) hybrids. Physiologia Plantarum 112: 470-477. ##McDonald, MB. 2000. Seed Priming. In: Black, M. and J. D. Bewley (Eds.), Seed Technology and its Biological Basis. Sheffield Academic Press, Sheffield, UK. pp: 287-325.##Peleg, Z., M. Reguera, E. Tumimbang, H. Walia and E. Blumwald. 2011. Cytokinin-mediated source ⁄sink modifications improve drought tolerance and increase grain yield in rice under water-stress. Plant Biotechnol. J. 8: 1-12.##Rehman, H., S. M. A. Basra and M. Farooq. 2011. Field appraisal of seed priming to improve the growth, yield, and quality of direct seeded rice. Turk. J. Agric. Forest. 35: 357-365.##Saeidi, M., F. Moradi, A. Ahmadi, K. Poostini and G. Najafian. 2006. Effect of exogenous application of ABA and CK at different stages of grain development on some physiological aspects of source and sink relationship in two bread wheat cultivars. Iran J. Crop Sci. 8) 3): 268-282. (In Persian with English abstract).##Walker, DJ., P. Romero, A. de Hoyos and E. Correal .2008. Seasonal changes in cold tolerance, water relations and accumulation of cations and compatible solutes in Atriplex halimus L. Environ. Exp. Bot. 64: 217-224.##Wingler, A., A. Scahewen, C. L. Richard, J. Peter and W. L. Paul-Quic. 1998. Regulation of leaf senescence by cytokinin, sugars and light. Plant Physiol. 116: 329-335.##Xu, G., J. Zhang, HM. Lam, Z. Wang and J. Yang. 2007. Hormonal changes are related to the poor grain filling in the inferior spikelets of rice cultivated under non-flooded and mulched condition. Field Crops Res. 101: 53-61.##Yang, J. C., J. Zhang, Z. Wang and Q. Zhu. 2003. Hormones in the grains in relation to sink strength and post anthesis development of spikelets in rice. Plant Growth Regul. 41: 185-195.##
2. Aboutalebian, M. A., F. Sharifzadeh, M. R. Jahansouz, A. Ahmadi, and M. R. Naghavi. 2008. The Effect of seed priming on germination, stand sstablishment and yield of wheat (Triticum aestivum L.) cultivars in three different climates of Iran. Iran. J. Field Crops Sci. 39(1): 145-154. (In Persian with English abstract).##Abdolrahmani, B., K. Ghassemi-Golezani, M. Valizadeh, V. Feizi-Asl, and A. R. Tvakoli. 2011. Effects of seed priming on seed vigor and grain yield of barley (Hordeum vulgare L. cv. Abidar) in rainfed conditions. Seed Plant Prod. J. 11(4): 337-352 (In Persian with English abstract). ##Abdolrahmani, B., K. Ghassemi-Golezani, M. Valizadeh and V. FeiziAsl. 2007. Seed priming and seedling establishment of barley (Hordeum voulgare). J. Food Agric. Environ. 5: 179-184.##Amal, M., E. Shraiy, and A. M. Hegazi. 2009. Effect of acetylsalicylic acid, indole-3-butyric acid and gibberellic acid on plant growth and yield of pea (Pisum sativum L.). J. Basic Appl. Sci. 3: 3514-3523.##Ashraf, M. Y., N. Azhar and M. Hussain. 2006. Indole acetic acid (IAA) induced changes in growth, relative water contents and gas exchange attributes of barley (Hordeum vulgare L.) grown under water stress conditions. Plant Growth Regul. 50:85-90.##Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts: polyphenol-oxidase in Beta vulgaris. Plant Physiol. 24: 1-15.##Bajguz, A. and A. Piotrowska. 2009. Conjugates of auxin and cytokinin. Phytochemistry. 70: 957-969##Bhatia, S. and R. Singh. 2002. Phytohormone-mediated transformation of sugars to starch in relation to the activities of amylases, sucrose-metabolising enzymes in sorghum grain. Plant Growth Regul. 00: 1-8.##Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye bonding. Analytical Biochem. 72: 248-254.##Brenner, M. L. and N. Cheikh. 1995. The role of phytohormones in photosynthate partitioning and seed filling. In: Davies P.J. (Ed.), Plant Hormones. Kluwer Academic Press, Dordrecht, Boston, London, pp. 649-670.##Davies, P. J. 1987. The Plant Hormones: Their Nature, Occurrence and Functions. In: P. J. Davies, P. J. (Ed.), Plant Hormones and Their Role in Plant Growth and Development. Martinus Nijhoff Publishers, The Netherlands, pp 1-11.##Dua, I. S. and S. N. Bhardwaj. 1979. Levels of endogenous growth regulators in wheat during early stages of grain setting and development. Indian J. Agric. Sci. 55: 622-627. ##Dubios, M. K. A. Gills, J. K. Hamilton, P. A. Rebers and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Annals Chem. 28(3): 350-356.##Ellis, R. H. 1992. Seed and seedling vigour in relation to crop growth and yield. Plant Growth Regul. 11: 249-255.##Farooq, M., S. M. A. Basra, H. Rehman and B. A. Saleem. 2008. Seed priming enhances the performance of late sown wheat (Triticum aestivum L.) by improving chilling. J. Agron. Crop Sci. 194: 55-60.##Ghorbani Javid, M., A. Soroushzadeh, S. A. M. Modarres Sanavy, I. Allahdadi and F. Moradi. 2011. Effects of the exogenous application of auxin and cytokinin on carbohydrate accumulation in grains of rice under salt stress. Plant Growth Regul. 65:305-313. ##Giri, G. S. and W. F. Schillinger. 2003. Seed priming winter wheat for germination, emergence and yield. Crop Sci. 43: 2135-2141. ##Gupta, N. K. S. Gupta, D. S. Shukla and P. S. Deshmukh. 2003. Differential responses of BA injection on yield and specific grain growth in contrasting genotypes of wheat (Triticum aestivum L.). Plant Growth Regul. 40: 201-205.##Hakoomat, A., I. Nadeem, S. Naeem, A. Shakeel and M. Athar. 2013. Seed priming improves irrigation water use efficiency, yield and yield components of late-sown wheat under limited water conditions. Turk. J. Agric. Forest. 37: 534-544. ##Harris, D., A. K. Pathan, P. Gothkar, A. Joshi, W. Chivasa and P. Nyamudeza. 2001. On-farm seed priming: using participatory methods to revive and refine a key technology. Agric. Sys. 69: 151-164.##Hashemi Dezfouli, A. And A. S. Marashi. 1995. Changes in photosynthetic material at flowering time and its effect on grain growth, yield and yield components of wheat. J. Agric. Sci. Technol. 9: 32-16. (In Persian with English abstract).##Havlova, M., P. I. Dobrev, V. Motyka, H. Storchova, J. Libus, J. Dobra', J. Malbeck, H. Gaudinova and R. Vankova. 2008. The role of cytokinins in responses to water deficit in tobacco plants over-expressing trans-zeatin O-glucosyltransferase gene under 35S or SAG12 promoters. Plant Cell Environ. 31: 341-353.##Herzog, H. 1982. Relation of source and sink during grain filling period in wheat and some aspects of the regulation. Physiol. Plant. 56: 155-160.##Khan, M. B., M. Gurchani, M. Hussain and K. Mahmood. 2010. Wheat seed invigoration by pre-sowing chilling treatments. Pak. J. Bot. 42: 1561-1566.##Liang, J., J. Zhang and X. Cao. 2001. Grain sink strength may be related to the poor grain filling of indica-japonica rice (Oryza sativa) hybrids. Physiologia Plantarum 112: 470-477. ##McDonald, MB. 2000. Seed Priming. In: Black, M. and J. D. Bewley (Eds.), Seed Technology and its Biological Basis. Sheffield Academic Press, Sheffield, UK. pp: 287-325.##Peleg, Z., M. Reguera, E. Tumimbang, H. Walia and E. Blumwald. 2011. Cytokinin-mediated source ⁄sink modifications improve drought tolerance and increase grain yield in rice under water-stress. Plant Biotechnol. J. 8: 1-12.##Rehman, H., S. M. A. Basra and M. Farooq. 2011. Field appraisal of seed priming to improve the growth, yield, and quality of direct seeded rice. Turk. J. Agric. Forest. 35: 357-365.##Saeidi, M., F. Moradi, A. Ahmadi, K. Poostini and G. Najafian. 2006. Effect of exogenous application of ABA and CK at different stages of grain development on some physiological aspects of source and sink relationship in two bread wheat cultivars. Iran J. Crop Sci. 8) 3): 268-282. (In Persian with English abstract).##Walker, DJ., P. Romero, A. de Hoyos and E. Correal .2008. Seasonal changes in cold tolerance, water relations and accumulation of cations and compatible solutes in Atriplex halimus L. Environ. Exp. Bot. 64: 217-224.##Wingler, A., A. Scahewen, C. L. Richard, J. Peter and W. L. Paul-Quic. 1998. Regulation of leaf senescence by cytokinin, sugars and light. Plant Physiol. 116: 329-335.##Xu, G., J. Zhang, HM. Lam, Z. Wang and J. Yang. 2007. Hormonal changes are related to the poor grain filling in the inferior spikelets of rice cultivated under non-flooded and mulched condition. Field Crops Res. 101: 53-61.##Yang, J. C., J. Zhang, Z. Wang and Q. Zhu. 2003. Hormones in the grains in relation to sink strength and post anthesis development of spikelets in rice. Plant Growth Regul. 41: 185-195.##
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Khamdi N, Nabipour M, Roshanfekr H, Rahnama A. Effect of seed priming and application of cytokinin and auxin on growth and grain yield of wheat (Triticum aestivum L.) under Ahvaz climatic conditions. علوم زراعی 2019; 21 (1) :31-44
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Volume 21, Issue 1 (Spring 2019 2019) Back to browse issues page
نشریه علوم زراعی ایران Iranian Journal of Crop Sciences
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